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The enzyme urease (EC 3.5.1.5) converts urea to ammonia and carbon dioxide. http://jcs.biologists.org/content/107/4/1073.full.pdf

  • The present invention relates to pharmaceutical compositions comprising a mixture of probiotics, prebiotics, and ammoniaphilic  bacteria with high urease activity, and/or sorbents with specific adsorption affinities for uremic toxins such as creatinine, uric acid, phenols, indoles, middle molecular weight molecules and inorganic phosphate along with a water sorbent, for use in the alleviation of uremia.
  • Bacillus pasteurii and Sporosarcina ureae are closely related soil bacteria, both of which have high avidity for urea.
  • These bacteria are non-pathogenic and safe.
  • Further, they grow well in high concentrations of ammonium ion and alkaline pH, which are present in the intestine, particularly in uremic conditions.
  • In Bacillus pasteurii and Sporosarcina ureae, no accumulation of ammonium occur and these organisms depend on passive diffusion of ammonia across the cell membrane.
  • Both B. pasteurii and S. ureae exhibit low affinities for ammonium, with Km values of 55.2 mM and 36.7 mM respectively.
  • In contrast to P. vulgaris, a pathogen which can only grow in neutral pH and low ammonium concentration of 2mM, B. pasteurii and S. ureae require high concentrations of ammonium (40 mM) and alkaline pH (Kaltwasser, Morsdorf G. H. Arch Microbiol 1989; 152(2): 125-31).
  • In addition, it is known that urease of Sporosarcina ureae has a specific activity of greater than 9300 mol of urea degradation per minute at a pH of 7.5
  • The methods for urea, ammonia, creatinine and uric acid quantitative analysis are commercially available diagnostic kits (Sigma, St. Louis, MO. Cat Nos. 535 and 171, respectively and Advanced Diagnostics, Inc.  Division of Inamco Group, South Plainfield, NJ. Cat Nos. 131 500 and CASO-50, respectively) (http://www.google.co.in/patents/CA2447376C?cl=en)
​​​Urinal odour eliminator and drain uric acid degrader 
  • The amount of urine produced depends on numerous factors including Room Temperature and other environmental factors like state of hydration, activities, age, weight and health.
  • In adult humans the average production is about 1 - 2 L per day.​

​URINSOLVE

​ABOUT URINE

  • Urine is liquid waste excreted by the kidneys and is produced by a process of filtration from blood.
  • This waste is eventually expelled from the body in a process known as urination
  • Healthy urine is a clear aqueous solution, varying in colour from dark yellow to colorless, depending on dilution.
  • The constituents besides water are Urea, Creatinine, Uric acid and Urochrome .
  • Urine also contains various inorganic ions, including sodium and chloride.
  • Lighter urine indicates higher water consumption.
  • Odor of urine can become worse when the urea in urine converts to noxious ammonia gas.​
URINE ANALYSIS
  • Color: yellow-amber to clear
  • Smell: Sweet or fruity odor due to the presence of ketones in the case of a diabetic patient
  • Generally fresh urine has a mild smell but aged urine has a stronger odor, similar to that of ammonia.
  • Acidity: pH in the range 4.6 - 8, 
  • High protein diets result in more acidic urine. 
  • Vegetarian diets generally result in more alkaline urine.
  • Density: Slightly greater than, 1.0.

Urine Analysis
Water:
94-96%
Urea (CON2H4 or
(NH2)2CO) (carbamide).
4.2-5.2 %
Creatinine
0.17-0.21%
Uric acid (C5H4N4O3.)
0.15-0.2 %
Phosphates 
0.13-.15%
Carbohydrates
Traces
Enzymes
Traces
Fatty acids
Traces
Hormones
Traces
Pigments
Traces
Sodium
Traces
Potassium
Traces
Chlorides
Traces
Magnesium 
Traces
Calcium  
Traces
Ammonia 
Traces
Sulphates 
Traces
Mucins
Traces
METHOD OF APPLICATION

In the case of Waterless Urinal System and Conventional Flushing Urinals:
  • Dilute 1 part URINSOLVE powder to 20 parts water and sprinkle or pour direct into urinal drain pipe for slow-flowing urinals.
  • In case of URINSOLVE tablet, dissolve 1 tablet in water and use similarly.
  • Simply apply URINSOLVE powder (1-2 gm / bowl) or 1 tablet / bowl, directly inside the bowl, behind toilet bowls, around sinks and basins and floor area, underneath the urinals or pour direct into urinal drain pipe for slow-flowing urinals daily at least once and the source of the odour is neutralized within a very short time..

In the case of well watered Urinal System:
  • Dilute 1 part URINSOLVE Powder to 20 parts water or 1 tablet in water and sprinkle liberally directly inside the bowls, behind toilet bowls, around sinks and basins and floor area underneath the urinals daily.

SAFETY
  • Never spray URINSOLVE directly on animals or humans.
  • Spray only on inanimate objects.​
​UNIQUE FEATURES OF URINSOLVE

  1. It doesn't just mask the odor, but contains an active odor counteracting agent.
  2. This is the step to an integrated approach to sanitation where human excreta is contained, sanitized and recovered for use in soil systems to enhance agricultural production.
  3. Deactivates the crystallized urine deposits rendering them inactive.
  4. Digests the source of protein odors such as urine, feces, milk, blood, and more.
  5. Breaks down the residues on which the bacteria feed off and contribute to the ongoing odor problems.
  6. Stop or reverse the yellow discoloration caused by urine contamination.

CONTENTS AND MODE OF APPLICATION:
  • The enzymes secreted by the microbes present in URINSOLVE breaks down the complex odorous substances into simple nutrients utilized by the microbes present in the URINSOLVE.
  • To achieve spontaneous results enzymes are used.
  • By the time the enzymes that are present in URINSOLVE initiate the process, the microbes will equip themselves to work in a sustained way  and can work for almost 5~15 days throughout the day and night irrespective of Temperature, pH, Hardness etc, if they are not killed by use of toxic chemicals. 
  • Microbes in URINSOLVE will form BIOFILM in the ducts and pipes and will tackle all the waste that come into contact.​
CITATIONS:
  • We have isolated and identified nine cultures of aerobic bacteria capable of growing on an elective medium containing uric acid as the only source of carbon, nitrogen, and energy. 
  • Four of these cultures were identified as Aerobacter aerogenes, two as Klebsiella pneumoniae and the remainder as Serratia killiensis, Pseudomonas aeruginosa and Bacillus species. 
  • Another culture identified as P. fluorescens required both glucose and uric acid for growth.
  • When 23 laboratory stock cultures were inoculated into the uric acid medium, A. aerogenes, B. subtilis, Mycobacterium phlei, P. aeruginosa, and S. marcescens were able to grow.
  • These five cultures also grew when the uric acid was replaced with adenine, guanine, hypoxanthine, xanthine, or allantoin, but growth was poor.
  • In all of these media, including the uric acid medium, addition of glucose along with the nitrogenous compounds yielded good growth.
  • Induction experiments demonstrated that the ability of A. aerogenes, K. pneumoniae, P. aeruginosa, P. fluorescens, S. kiliensis, S. marcescens, B. subtilis and Bacillus sp. to degrade uric acid is an induced property.
  • Of these organisms, only Bacillus sp. accumulated a small amount of intracellular uric acid. (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC252350/)